A range of modern microscopes is available for routine research use.
Each is fitted with a high quality digital camera for image acquisition.
- Olympus BX50 Fluorescence (upright microscope)
- Olympus AX70 Fluorescence (upright microscope)
- Olympus IX71 Fluorescence & Contrast (inverted microscope)
- Olympus BX50 Brightfield (upright microscope)
- SZX10 Stereo Microscope / Macroscope (dissecting scope with reflected light illumination)
Image Viewing, Processing and Analysis of Fluorescence Images
Images acquired using the Fluorescence and Brightfield microscopes within Flinders Microscopy will usually be in .tif fomat (TIFF files). This is the recommended format for microscopy images. TIFF files can be opened via picture viewing software on your own computer.
ImageJ (http://rsb.info.nih.gov/ij) and Cell Profiler (www.cellprofiler.org) are two freely downloadable software that can be used to carry out a wide range of image processing and analysis functions.
Adobe Photoshop (www.adobe.com/products/photoshop.html) can be used to ‘false colour’ images, merge images and adjust brightness and contrast with a high degree of precision.
Upon successfully completed training, users are given access to the facility and the respective instruments for which they have been trained. All equipment use must be booked, and user fees apply.
User fees are kept to a minimum realistic rate. Funds from user fees go back into the Facility to cover repairs, parts replacement and equipment upgrades.
Brightfield and fluorescence microscope user fee charges are invoiced to laboratory supervisors bi-annually.
Please refer to the current table of User Fees (PDF 13KB) for more information.
All usage of microscopes and associated equipment must be booked by using the online Calcium Booking Calendar.
The calendars may be viewed by everyone. However, the ability to book specific equipment will only be enabled after successfully completed training with Flinders Microscopy Management Personnel.
Useful Resources and Link
Fluorescence imaging principles and virtual instrument / simulator tutorials:
Software to analyse microscopic images
Tutorials on the basics of fluorescence, excitation and emission – Life Technologies
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