Cell Screen SA
High Throughput Screening Facility
Cell Screen SA (CeSSA) is a high throughput screening facility that provides several services.
- RNAi and compound library screening
- Custom library screening
- Advice on screen design and development
- Assay development
- High content image analysis
- Data analysis
- Any experiment, small or large, that utilises the unique equipment at CeSSA
- All assay protocols used at CeSSA are assessed for accuracy, variability and appropriate screening metrics (Signal/Background, Z-Prime)
All fully-charged work done at CeSSA comes with fully reported and publication quality methods, clear data presentation and, where applicable, analysis.
High throughput screening projects normally require substantial assay development and testing of protocols to ensure they meet the standards needed for quality results.
CeSSA has several instruments that make up the core of the facility.
Instruments at CeSSA:
- Operetta®: High-content imaging system for high-throughput imaging and image analysis. Brightfield, fluorescence, confocal and non-confocal microscope.
- Ensight: Multimode plate reader for absorbance, fluorescence, luminescence, time-resolved fluorescence and, uniquely, AlphaLISA® assay measurement.
- Janus®: Liquid handler for complex pipetting procedures. Range from nano to microliters. 8-tip Varispan™ for multi-tipped processing from tubes to vials to plates. Also has a 96-tip Modular Dispense Technology (MDT) dispense arms for liquid handling on 96 and 384 well plates.
- Biotek: Plate aspiration and dispense. Dispenser for variable volume dispensing of liquid through columns or rows.
- Cell:ExplorerTM(plate::handler): Robotic arm for movement of plates between all instruments allowing automated workflows for high-throughput screening applications or time-course imaging.
- We also have ample bench space, two CO2 and temperature controlled incubators, a biohazard hood and laminar flow cabinet.
All instruments can be used integrated for complete automation but can also be used separately.
Due to the complexity of our instruments, their use requires extensive training and experience and their use is retricted to CeSSA staff.